Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

14.4K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
14.4K
Membrane Domains01:18

Membrane Domains

6.9K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
6.9K
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

10.0K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomimetic M2 exosome-based dual-drug nanoplatform for combined immunomodulation and bone protection in rheumatoid arthritis.

Bioactive materials·2026
Same author

A G-Quadruplex-Activated Near-Infrared Chemiluminescent Probe for In Situ Hepatic Imaging of the Hepatitis C Virus Genome.

Angewandte Chemie (International ed. in English)·2026
Same author

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
Same author

A novel method to quantify viable Enterococcus faecium during feed manufacturing.

Poultry science·2026
Same author

Reference Ranges of Maximum Diameter and Volume of the Fetal Large Intestine During the Second-to Third-Trimester: An MRI Study.

Prenatal diagnosis·2026
Same author

Understanding the kinetics and mechanism of heat-induced aggregation of chickpea globulin.

Food chemistry·2026

Related Experiment Video

Updated: Jan 7, 2026

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems
07:10

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Published on: February 23, 2024

1.6K

Inner Leaflet-Anchored Multiplexed DNA Toolkits for Dynamic Tracking and Programmable Regulation of Cell Membrane

Yuanyuan Chen1, Beiling Chen1, Xiaohan Zhou1

  • 1Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo and Biosensing, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.

Journal of the American Chemical Society
|January 2, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed liposome fusion-based transport (LiFT) to deliver multiplexed DNA tools to the inner cell membrane leaflet. This enables precise monitoring and control of inner membrane processes like ion transport and signal transduction.

More Related Videos

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.8K
Simple, Affordable, and Modular Patterning of Cells using DNA
08:59

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

4.6K

Related Experiment Videos

Last Updated: Jan 7, 2026

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems
07:10

Author Spotlight: Photo Switchable Protein Recruitment for Reversible Patterning in Artificial Cellular Systems

Published on: February 23, 2024

1.6K
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
10:49

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Published on: March 5, 2017

13.8K
Simple, Affordable, and Modular Patterning of Cells using DNA
08:59

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

4.6K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Inner membrane leaflet biological processes are crucial for cellular function.
  • Efficient delivery of multiplexed chemical tools to the inner leaflet for biomolecule engagement with stoichiometric control is challenging.
  • Previous studies primarily focused on the outer membrane leaflet, leaving the inner leaflet under-explored.

Purpose of the Study:

  • To develop a method for precise integration of multiplexed DNA toolkits onto the inner membrane leaflet.
  • To enable dynamic monitoring and programmable manipulation of inner membrane processes.
  • To investigate transmembrane transport and signal transduction at the inner leaflet.

Main Methods:

  • Liposome fusion-based transport (LiFT) for delivering DNA toolkits to the inner membrane leaflet.
  • Incorporation of distinct, bioorthogonal DNA sequences with high efficiency and defined orientation.
  • Development of membrane-anchored multiplexed sensors for ion dynamics mapping.
  • Utilizing sequence-programmable DNA-enzyme ligation for protein recruitment and signaling activation.
  • Employing strand-displacement reactions and photolabile chemistry for signaling modulation.

Main Results:

  • Successful integration of multiplexed DNA toolkits onto the inner membrane leaflet via LiFT.
  • High efficiency and defined orientation of bioorthogonal DNA sequences achieved.
  • Dynamic monitoring of transmembrane ion dynamics, including proton gradients and Na+/K+ fluxes.
  • First demonstration of DNA-based programmable control of inner leaflet signaling via protein translocation.
  • Modulation of inner leaflet signaling cascades using sequence inputs or light irradiation.

Conclusions:

  • LiFT provides a robust method for delivering functional DNA toolkits to the inner membrane leaflet.
  • Multiplexed DNA tools enable precise tracking and regulation of inner leaflet biological activities.
  • This approach opens new avenues for studying and manipulating cellular signaling and transport at the inner membrane.