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Published on: August 14, 2019
Picolinic Acid Derivatives and Biosynthetic Pathway from Epicoccum sorghinum SDU-F549
Liuhai Chen1, Changfen Li1, Feng Li1
1State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Key Laboratory of Chemical Biology (Ministry of Education), Shandong Basic Science Research Center (Pharmacy), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, People's Republic of China.
Abstract:
Two new picolinic acid derivatives, ethylfusaric acid A (1) and ethylfusaric acid B (2), along with two known picolinic acid derivatives (3-4), were isolated from the culture of Rehmannia glutinosa endophytic fungus Epicoccum sorghinum SDU-F549. The chemical structures of the compounds were elucidated by the combination of 1D and 2D nuclear magnetic resonance spectroscopy and electronic circular dichroism calculations. Compounds 1-4 exhibited antibacterial activity against a panel of Staphylococcus aureus strains, with MIC values ranging from 16 to 32 μg/mL. In contrast, they showed no significant cytotoxic activity against a range of human tumor cell lines at 10 μM. Furthermore, bioinformatic analysis revealed that the bty biosynthetic gene cluster in E. sorghinum SDU-F549 is responsible for assembling the picolinic acid derivatives, enabling us to propose the biosynthetic pathways of compounds 1-4.
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