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Related Experiment Video

Updated: Jan 7, 2026

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

Imaging Dynamic Subcellular Organization at High Spatiotemporal Resolution.

Francesca W van Tartwijk^1,2, Liuba Dvinskikh¹, Edward N Ward¹

¹Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom;

Annual Review of Biophysics

|January 2, 2026

Summary

This summary is machine-generated.

Recent advances in fluorescence microscopy and computational methods enhance our ability to study intracellular compartment dynamics. These imaging innovations offer deeper insights into cellular organization and function in health and disease.

Keywords:

fluorescence microscopy light-sheet fluorescence microscopy live-cell imaging organelle dynamics subcellular organization super-resolution microscopy

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Last Updated: Jan 7, 2026

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Published on: August 16, 2021

Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Intracellular compartment organization is crucial for cellular function, health, and disease.
Fluorescence microscopy is key for observing organelle morphology and dynamics.
Current microscopy techniques face limitations in capturing diverse spatiotemporal scales.

Purpose of the Study:

To review recent advancements in high-resolution fluorescence microscopy and computational methods.
To critically evaluate how these advances overcome existing limitations in imaging.
To illustrate the impact of new technologies on understanding cellular organization and function.

Main Methods:

Review of recent developments in high-resolution fluorescence microscopy.
Analysis of associated computational methods for image processing and analysis.
Illustration using biological examples of organelle dynamics.

Main Results:

New fluorescence microscopy techniques and computational tools address limitations in resolution, speed, and signal-to-noise ratio.
These advances enable better observation of organelle dynamics across various spatiotemporal scales.
Biological examples demonstrate enhanced understanding of cellular organization and function.

Conclusions:

Technological innovations in fluorescence microscopy are significantly improving the study of subcellular processes.
Further advancements hold potential for deeper insights into dynamic cellular mechanisms.
This field is critical for understanding processes in both health and disease.