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Updated: Jun 7, 2026
Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
Published on: September 10, 2013
A dual-responsive ratiometric fluorescence sensor for highly sensitive detection of heparin with Eu CPs and
Yan-Zhao Fang1, Yan Zhang1, Lin-Xin Du1
1Jiangxi Provincial Key Laboratory of Organic Functional Molecules; Institute of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.
Abstract:
In this study, a new dual-responsive ratiometric fluorescence nanoprobe was rationally designed for heparin (Hep) detection. The negatively charged europium-based coordination polymers (Eu CPs) and positively charged fluorescent polydopamine nanoparticles (FPDA NPs) could assemble via electrostatic interaction, meanwhile, the fluorescence of these both reduced slightly owing to self-quenching (SQ) and inner-filter effect (IFE), respectively. Upon addition of Hep, the Eu CPs emission at 615 nm was markedly quenched due to electron transfer and SQ, while the FPDA NPs emission at 465 nm was enhanced marginally owing to mitigation of the IFE. These opposing signal changes yielded a robust ratiometric readout (I₄₆₅/I₆₁₅) for Hep detection. The probe showed a widely linear response over 0-16 μg/mL with a detection limit as low as 3.58 ng/mL. Furthermore, high sensitivity and accuracy of the probe enabled accurate analysis of Hep in human serum with favorable recoveries, highlighting its potential for biomedical analysis and clinical diagnostics.
