Engineered core-shell nanocomposite fibres incorporating bio-ceramics and bioactive molecules for wound repair

Skin plays a major role in protecting the body from external injuries and contaminants. Despite the self-healing mechanisms of the body, wound healing has several limitations, such as being time-consuming, leading to scar formation, and susceptibility to infections. In this study, a novel core-shell nanofibre membrane was designed to protect wounds and prevent secondary trauma, thereby enhancing the wound healing process. A core-shell nanofibre membrane was prepared using polycaprolactone (PCL) as the core polymer loaded with astaxanthin (ASTX) and bioglass (BG), while the shell was made from polylactic acid (PLA) containing nanohydroxyapatite (nHA) to support faster wound healing. The surface structure, morphology, and hydrophilicity of the fibres were extensively characterised. The analysis revealed uniform, well-organised, interconnected core-shell nanocomposite fibres ideal for cell adhesion and growth. In vitro studies have demonstrated enhanced cell viability and wound closure in mouse L929 fibroblast cells. Immune response studies on test membranes loaded with ASTX, BG, and nHA revealed strong anti-inflammatory and antibacterial activities against Gram-positive and Gram-negative bacteria. In vivo studies indicated favourable cellular responses and superior wound healing potential of membranes incorporated with ASTX, BG and a higher concentration of nHA. These findings highlight the potential of core-shell nanofibre membranes as an innovative wound dressing for full-thickness skin injuries, showing significant promise for biomedical applications, especially in wound healing treatments.