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Cannabidiol Enhances SIRT1 and Autophagy for the Maintenance of Human Mesenchymal Stem Cells

Phongsakorn Chueaphromsri1, Phongsakorn Kunhorm1, Areechun Sotthibundhu2

  • 1Laboratory of Cell-Based Assays and Innovations, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.

In Vivo (Athens, Greece)
|January 2, 2026
PubMed
Abstract

Insights

Cannabidiol (CBD) delays aging in mesenchymal stem cells (MSCs) by boosting Sirtuin 1 (SIRT1) and autophagy. This preserves MSC stemness and function, offering potential for regenerative medicine applications.

Area of Science:

  • Stem cell biology
  • Molecular gerontology
  • Pharmacology

Background:

  • Mesenchymal stem cells (MSCs) are crucial for treating degenerative diseases.
  • Cellular aging limits MSC therapeutic efficacy during in vitro cultivation.
  • Identifying strategies to maintain MSC stemness and delay senescence is vital.

Purpose of the Study:

  • To investigate if cannabidiol (CBD) can delay MSC aging.
  • To explore CBD's effect on Sirtuin 1 (SIRT1) and autophagy.
  • To assess CBD's potential to preserve MSC stemness and function.

Main Methods:

  • MSCs were treated with CBD.
  • Assessed cell viability, doubling time, and key gene/protein expression.
  • Evaluated senescence markers (SA-β-gal), telomere length, and telomerase activity.

Main Results:

  • CBD significantly upregulated SIRT1 and autophagy markers in MSCs.
  • CBD preserved MSC stemness by promoting SOX2 deacetylation via SIRT1.
  • CBD delayed senescence, reduced SA-β-gal activity, and maintained proliferation and telomere function.

Conclusions:

  • CBD promotes MSC stemness and delays cellular senescence.
  • CBD's effects are potentially mediated by activating SIRT1 and autophagy.
  • CBD shows promise for preserving MSC function in regenerative medicine.
Keywords:
CannabidiolSIRT1autophagymesenchymal stem cellsstemness

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