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Innovative Herbal-Based Decellularization of Pericardium for Advanced Polymeric Skin Substitutes.
Azadeh Nochalabadi1, Mozafar Khazaei1,2, Sepideh Kadivarian3
1Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Artificial Organs
|January 2, 2026
Summary
Acanthophelium extract (ACP) offers a safer, biocompatible alternative for decellularizing sheep pericardium scaffolds. Combining ACP with SDS yields effective skin engineering scaffolds with preserved extracellular matrix and enhanced antibacterial properties.
Area of Science:
- Tissue engineering
- Regenerative medicine
- Biomaterials
Background:
- Decellularization techniques are crucial for creating biological scaffolds in regenerative medicine.
- Chemical agents like SDS can cause cytotoxicity, necessitating safer alternatives.
- Herbal extracts present a promising biocompatible option for scaffold preparation.
Purpose of the Study:
- To evaluate Acanthophelium extract (ACP) for decellularizing sheep pericardium.
- To compare ACP's efficacy with SDS for skin engineering applications.
- To investigate the synergistic effects of ACP and SDS in scaffold preparation.
Main Methods:
- Sheep pericardium tissues were decellularized using varying concentrations of ACP and SDS, individually and in combination.
- Evaluated scaffolds through cell staining, biocompatibility assays (MTT), hemolysis, blood clotting index (BCI), SEM, ATR-FTIR, mechanical testing, contact angle, and antibacterial assays.
Main Results:
- ACP and SDS combinations achieved complete cell removal while preserving the extracellular matrix.
- All scaffolds exhibited over 90% biocompatibility.
- ACP-based scaffolds demonstrated reduced hemolysis, favorable BCI, preserved protein structure, increased porosity, and enhanced hydrophilicity.
- The 10% ACP + 0.1% SDS group showed the highest antibacterial activity.
Conclusions:
- ACP extract is an effective and biocompatible agent for pericardial decellularization.
- Combining ACP with a low concentration of SDS produces balanced scaffolds suitable for skin engineering.
- These novel scaffolds possess desirable properties, including enhanced biocompatibility and antibacterial effects.
