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Related Experiment Video

Updated: Jun 15, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

Hydrogel based substrate controllable release for lateral flow assay with automatic signal amplification.

Jingyang Jiang^1,2, Yue Sun^1,2, Alberta Osei Barimah^1,2

¹State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Road 1800, Wuxi, 214122, PR China.

Mikrochimica Acta

|January 3, 2026

Summary

A novel hydrogel-integrated lateral flow assay (HG-LFA) platform simplifies detection by automatically releasing a substrate. This enhanced sensitivity and accuracy are ideal for point-of-care testing applications.

Keywords:

Delayed substrate release Hydrogel Lateral flow assay Nanozyme SARS-CoV-2 virus

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Last Updated: Jun 15, 2026

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Area of Science:

Biomedical Engineering
Assay Development
Point-of-Care Diagnostics

Background:

Conventional lateral flow assays (LFAs) have limited sensitivity for trace targets.
Nanozyme-based LFAs improve detection but add procedural complexity.
Current methods often require manual substrate addition, complicating the assay.

Purpose of the Study:

To develop a simplified, highly sensitive lateral flow assay (LFA) platform.
To overcome the limitations of conventional and nanozyme-based LFAs.
To create a hydrogel-integrated LFA (HG-LFA) for enhanced, automated signal amplification.

Main Methods:

Incorporated a sodium alginate (NaAlg) hydrogel network on a glass fiber membrane.
Encapsulated the chromogenic substrate 3,3'-diaminobenzidine (DAB) within the hydrogel.
Utilized a SARS-CoV-2 E nucleic acid target for performance evaluation.

Main Results:

Achieved a limit of detection (LOD) of 0.146 nM for SARS-CoV-2 E nucleic acid.
Demonstrated a 25-fold improvement over conventional LFAs without signal amplification.
Observed signal enhancement comparable to manual substrate addition, with automatic release.

Conclusions:

The hydrogel-integrated LFA (HG-LFA) platform offers automatic, enhanced signal amplification with low background.
The HG-LFA shows high accuracy and stability in biological matrices.
This facile strategy is well-suited for high-performance point-of-care testing (POCT).