A one-shot approach for serum vancomycin assessment using fluorescence-based molecularly imprinted polymer...

Area of Science:

• Nanotechnology
• Analytical Chemistry
• Biomedical Engineering

Background:

• Accurate vancomycin (VA) monitoring is crucial for therapeutic efficacy and preventing toxicity.
• Existing methods for VA detection can be time-consuming and require complex procedures.
• Development of rapid, sensitive, and selective assays for VA is highly desirable.

Purpose of the Study:

• To develop novel fluorescence-based molecularly imprinted polymer nanoparticles (MIP NPs) for sensitive vancomycin (VA) detection.
• To utilize thionine as both a functional monomer and an internal fluorescent probe for direct readout.
• To establish a rapid and selective assay for VA quantification in serum samples.

Main Methods:

• Preparation of MIP NPs incorporating fluorescent thionine within the polymer matrix.
• Utilizing the fluorescence quenching effect of vancomycin binding to MIP NPs for detection.
• Development of a fluorescence spectrometry assay for VA quantification in serum.

Main Results:

• The developed MIP NPs assay showed a linear response for VA from 1.55 to 100 mg/L with a low limit of detection (0.46 mg/L).
• High recovery rates (97-115%) and good within-run precision (2.7-6.2%) were achieved.
• The assay demonstrated high selectivity for VA over other common drugs and acceptable accuracy compared to a standard immunoassay, yielding results within 10 minutes.

Conclusions:

• Thionine-based MIP NPs provide a sensitive, selective, and rapid platform for vancomycin detection.
• The developed fluorescence assay offers a promising alternative to conventional methods for therapeutic drug monitoring.
• The unique properties of these MIP NPs suggest potential for broader applications in selective material development.