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LowLoad-qPCR as a novel clinical strategy for detecting low-load bacteremia
Paula Gómez Estévez1,2, José Miguel Cisneros1,2,3,4, José Antonio Lepe1,2,3,5
1Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Av. Manuel Siurot s/n 41013, Seville, Spain.
Abstract:
The primary obstacle in detecting bloodstream infections is their often extremely low bacterial concentration. To accurately detect such infections, we developed a quantitative PCR assay, named LowLoad-qPCR, based on the combination of increased input DNA by random priming amplification method and the use of multiple probes sharing a single fluorophore. Here, we detail the key improvements over conventional qPCR and evaluate its performance using blood samples.
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