Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.9K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.9K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.6K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.6K
Centrosome Duplication02:25

Centrosome Duplication

4.8K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.8K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.3K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.3K
Centrioles and Centrosomes01:13

Centrioles and Centrosomes

5.1K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
5.1K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

3.9K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laryngopharyngeal Symptoms and Laryngopharyngeal Reflux Disease: Interdisciplinary Considerations and Management.

Neurogastroenterology and motility·2026
Same author

Nuclear blebs are composed of variable chromatin states but consistently enrich transcription initiation relative to elongation.

bioRxiv : the preprint server for biology·2026
Same author

Human dynein-dynactin is a fast processive motor in living cells.

eLife·2026
Same author

Grip it and rip it.

Nature chemical biology·2026
Same author

Changes in nuclear and actin mechanics from G1 to G2 affect nuclear integrity.

Journal of cell science·2026
Same author

Topoisomerase I inhibition suppresses nuclear blebbing via RNA Pol II stalling and nuclear stiffening.

bioRxiv : the preprint server for biology·2026
Same journal

Disentangling the response to lysosomal damage.

Journal of cell science·2026
Same journal

The force, form and function of the nucleus.

Journal of cell science·2026
Same journal

The nucleus-vacuole junction at a glance.

Journal of cell science·2026
Same journal

Loss of INPP5E affects photoreceptor outer segment membrane biogenesis in iPSC-derived human retinal organoids.

Journal of cell science·2026
Same journal

Brinker regulates reciprocal outcomes of BMP signal between stem cells and differentiating cells.

Journal of cell science·2026
Same journal

Primary cilium disassembly - from mechanisms to roles in physiology and disease.

Journal of cell science·2026
See all related articles

Related Experiment Video

Updated: Jan 7, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.6K

CTCF maintains centromere function and mitotic fidelity.

Erin Walsh1, Thomas Laskarzewski1,2, Thomas J Maresca1,2

  • 1Biology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.

Journal of Cell Science
|January 5, 2026
PubMed
Summary
This summary is machine-generated.

The study reveals that CTCF protein is crucial for maintaining centromere function during cell division (mitosis). Its degradation leads to mitotic failure and abnormal nuclear shapes, highlighting its essential role in cell division accuracy.

Keywords:
CENP-ECTCFCentromereCohesinMitosisNuclear shape

More Related Videos

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.3K
Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

2.2K

Related Experiment Videos

Last Updated: Jan 7, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.6K
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.3K
Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

2.2K

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Mitosis requires accurate genome segregation.
  • CTCF (CCCTC-binding factor) is a chromatin protein with an unknown role in mitosis.
  • Previous studies indicated CTCF knockdown causes mitotic failure.

Purpose of the Study:

  • To elucidate the specific role of CTCF in mitosis.
  • To investigate the mechanism by which CTCF influences mitotic progression and centromere function.

Main Methods:

  • Utilized a CRISPR auxin-inducible degron system for rapid CTCF degradation.
  • Employed immunofluorescence microscopy to analyze mitotic spindle organization and centromere function.
  • Assessed nuclear morphology post-mitosis.

Main Results:

  • CTCF degradation resulted in increased mitotic failure and abnormal post-mitotic nuclear shapes.
  • Key centromere functions were disrupted, including increased intercentromere distances and a disorganized metaphase plate.
  • Observed phenotypes were similar to those seen with cohesin disruption, suggesting a role in centromere integrity.

Conclusions:

  • CTCF is essential for maintaining centromere function during mitosis.
  • CTCF plays a critical role in ensuring accurate chromosome segregation and proper post-mitotic nuclear formation.
  • CTCF acts as a key factor in the maintenance of centromere integrity and successful cell division.