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Updated: Jan 7, 2026
05:35
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
Published on: March 3, 2016
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CTCF maintains centromere function and mitotic fidelity
Erin Walsh1, Thomas Laskarzewski1,2, Thomas J Maresca1,2
1Biology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA.
Journal of Cell Science
|January 5, 2026
Summary
The study reveals that CTCF protein is crucial for maintaining centromere function during cell division (mitosis). Its degradation leads to mitotic failure and abnormal nuclear shapes, highlighting its essential role in cell division accuracy.
Area of Science:
- Cell Biology
- Genetics
- Molecular Biology
Background:
- Mitosis requires accurate genome segregation.
- CTCF (CCCTC-binding factor) is a chromatin protein with an unknown role in mitosis.
- Previous studies indicated CTCF knockdown causes mitotic failure.
Purpose of the Study:
- To elucidate the specific role of CTCF in mitosis.
- To investigate the mechanism by which CTCF influences mitotic progression and centromere function.
Main Methods:
- Utilized a CRISPR auxin-inducible degron system for rapid CTCF degradation.
- Employed immunofluorescence microscopy to analyze mitotic spindle organization and centromere function.
- Assessed nuclear morphology post-mitosis.
Main Results:
- CTCF degradation resulted in increased mitotic failure and abnormal post-mitotic nuclear shapes.
- Key centromere functions were disrupted, including increased intercentromere distances and a disorganized metaphase plate.
- Observed phenotypes were similar to those seen with cohesin disruption, suggesting a role in centromere integrity.
Conclusions:
- CTCF is essential for maintaining centromere function during mitosis.
- CTCF plays a critical role in ensuring accurate chromosome segregation and proper post-mitotic nuclear formation.
- CTCF acts as a key factor in the maintenance of centromere integrity and successful cell division.
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