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Updated: Jan 7, 2026
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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
Published on: April 17, 2017
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Lipid Droplet-Localized Spindle Apparatus Coiled-Coil Protein 1 Regulates Lipid Droplet Distribution
Honggang Su1,2, Huimin Pan1,2, Yaqiang Liu1,2
1State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|January 5, 2026
Summary
Spindle apparatus coiled-coil protein 1 (SPDL1-L) regulates intracellular lipid droplet (LD) transport. This protein drives LD clustering at the microtubule-organizing center, impacting cell division and lipid metabolism.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Lipid droplets (LDs) are crucial for cellular energy and lipid metabolism.
- The precise roles of LD-associated proteins in LD transport and cellular function are not fully understood.
Purpose of the Study:
- To identify key regulators of intracellular lipid droplet transport.
- To elucidate the function of spindle apparatus coiled-coil protein 1 (SPDL1-L) in LD dynamics.
Main Methods:
- Identification of SPDL1-L as an LD-localized protein.
- Analysis of SPDL1-L localization mechanisms (hydrophobic region, basic residues).
- Investigation of SPDL1-L's effect on LD clustering and nuclear morphology during cell division.
Main Results:
- SPDL1-L is identified as a novel regulator of intracellular LD transport.
- SPDL1-L mediates LD clustering at the microtubule-organizing center (MTOC) via dynein-dependent transport.
- SPDL1-L promotes the formation of toroidal nuclei during cell division.
Conclusions:
- SPDL1-L plays a significant role in governing LD dynamics and intracellular transport.
- SPDL1-L's function is linked to dynein-mediated minus-end transport and affects cellular organization.
- Findings offer new insights into LD regulation and their impact on cell division.
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