Development and Validation of RP-HPLC Method for trans-Resveratrol in HPβCD-Loaded Stealth Liposomes: Stability and...

Area of Science:

• Pharmaceutical Sciences
• Drug Delivery Systems
• Analytical Chemistry

Background:

• trans-Resveratrol (trans-RES) isomers, trans- and cis-resveratrol, exhibit structural similarities making separation challenging.
• trans-RES stability is compromised by isomerization to the inactive cis-form, necessitating improved formulation strategies.
• Enhancing trans-RES solubility and stability is crucial for its therapeutic applications.

Purpose of the Study:

• To develop and validate a method for quantifying trans-RES complexed with hydroxypropyl-β-cyclodextrin (HPβCD) in stealth liposomes.
• To create a dual-delivery system combining HPβCD inclusion complexes with stealth liposomes for enhanced trans-RES delivery.
• To evaluate the stability and release profile of trans-RES within the developed liposomal formulation.

Main Methods:

• Optimized a reversed-phase high-performance liquid chromatography (RP-HPLC) method for trans-RES quantification.
• Utilized an isocratic mobile phase (water:acetonitrile, 65:35 v/v) with UV detection at 306 nm.
• Formulated stealth liposomes incorporating HPβCD-trans-RES inclusion complexes (F1-F5) and assessed release kinetics.

Main Results:

• The RP-HPLC method demonstrated excellent linearity (R² = 0.9997), precision (RSD < 2%), accuracy (98-102% recovery), and sensitivity (LOD 0.96 μg/mL, LOQ 2.91 μg/mL).
• High resolution (Rs > 16.5) was achieved between trans- and cis-resveratrol isomers.
• Liposomal formulation F5 exhibited sustained trans-RES release (49.8% at 12 h) following the Korsmeyer-Peppas model (R² > 0.90) and protected trans-RES from UV-induced isomerization.

Conclusions:

• A robust and validated RP-HPLC method enables accurate quantification of trans-RES in HPβCD-loaded stealth liposomes.
• The developed dual-delivery system effectively enhances trans-RES solubility, stability, and provides sustained release.
• This formulation strategy offers a promising approach for improving the bioavailability and therapeutic efficacy of trans-RES.