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Effects of Low-Dose Carbon Monoxide on Antibiotic Efficacy
Alana White1, Yasmeen Rasasi2, Stuart Gordon2
1Department of Chemistry and Biochemistry, Presbyterian College.
Abstract:
The impact of intestinal gases, including gasotransmitters, on antibiotic efficacy is severely understudied. This study assessed the effects of low-dose CO (85 µg/g) on the efficacy of various antibiotics in low oxygen conditions using the Kirby-Bauer method against E. coli BW20767/pRL27. Preliminary results showed that exposure to CO exerts variable effects on antibiotic efficacy. This indicates that CO exerts its effects not only through modulation of E. coli 's respiratory chain, but may also involve additional, as-yet unidentified targets independent of terminal oxidase binding. We also observed that E. coli endogenously produces CO as it switches to anaerobic metabolism.
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