Related Experiment Video
Updated: Jan 7, 2026
13:24
An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
20.5K
Complete genome sequence of Arthrobacter globiformis bacteriophage Powelldog
Zeel Desai1, Nathaniel Foret1, Skylar L Godwin1
1Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, North Carolina, United States.
Micropublication Biology
|January 5, 2026
Summary
Bacteriophage Powelldog was discovered in Charlotte, NC dog park soil. Its genome contains 91 genes, including a unique fusion protein, offering insights into phage biology.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Bacteriophages are viruses that infect bacteria and play crucial roles in microbial ecosystems.
- Isolation and characterization of novel bacteriophages contribute to understanding viral diversity and evolution.
Purpose of the Study:
- To isolate and characterize a novel bacteriophage from a canine-associated environment.
- To determine the genomic features of the isolated bacteriophage, Powelldog.
Main Methods:
- Isolation of bacteriophage Powelldog using Arthobacter globiformis NRRL B-2880 as the host.
- Genomic DNA extraction and sequencing.
- Bioinformatic analysis of the bacteriophage genome.
Main Results:
- Bacteriophage Powelldog was successfully isolated from dog park soil.
- The genome size is 55,541 base pairs and contains 91 putative protein-coding genes.
- A unique putative major capsid protease fusion protein was identified within the genome.
Conclusions:
- Bacteriophage Powelldog represents a novel phage with potential applications in phage therapy or as a research tool.
- The unidirectional gene transcription and unique protein-coding genes highlight novel aspects of its biology.
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