Related Experiment Video

Updated: May 7, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.5K

The differences in microbial composition may be an important cause of pancreatic pseudocyst infection-an

Yaoting Li1, Yongzhan Zhao1, Senlin Hou1

  • 1Department of Biliopancreatic Endoscopic Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Frontiers in Microbiology
|January 5, 2026
PubMed
Abstract

Insights

Microbial differences in pancreatic pseudocyst fluid impact infection risk. Higher levels of specific bacteria like Klebsiella and Streptococcus were found in infected pseudocysts, suggesting a link to postoperative infections.

Area of Science:

  • Gastroenterology
  • Microbiology
  • Infectious Diseases

Background:

  • Limited research exists on microorganisms in pancreatic pseudocyst infections.
  • Pancreatic pseudocysts are fluid collections that can become infected, leading to complications.

Purpose of the Study:

  • To investigate the microbiological distinctions between infected and non-infected pancreatic pseudocysts.
  • To identify specific microbial signatures associated with pancreatic pseudocyst infections.

Main Methods:

  • An observational cohort study included 37 patients undergoing endoscopic ultrasound (EUS)-guided drainage for pancreatic pseudocysts.
  • Patients were categorized into infected and non-infected groups post-drainage.
  • Cyst fluid underwent microbial sequencing to analyze bacterial composition.

Main Results:

  • Clinical features were similar between infected and non-infected groups.
  • No significant difference in alpha diversity of microbial communities was observed.
  • Beta diversity showed significant differences, with higher relative abundance of Klebsiella, Streptococcus, Collinsella, Phascolarctobacterium, and Megamonas in infected pseudocysts.

Conclusions:

  • Microbial composition of pancreatic pseudocyst fluid may influence postoperative infection development.
  • Elevated levels of specific genera (Klebsiella, Streptococcus, Collinsella, Phascolarctobacterium, Megamonas) are associated with infected pseudocysts.
  • Further research is warranted to confirm these findings and their clinical implications.
Keywords:
16sRNAEUSinfectionmicroorganismpancreatic pseudocyst

More Related Videos

Characterization and Functional Prediction of Bacteria in Ovarian Tissues10:12

Characterization and Functional Prediction of Bacteria in Ovarian Tissues

Published on: October 23, 2021

3.2K
Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes03:53

Growing a Cystic Fibrosis-Relevant Polymicrobial Biofilm to Probe Community Phenotypes

Published on: April 19, 2024

1.0K

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

Overview
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Microbiota of the Respiratory Tract01:29

Microbiota of the Respiratory Tract

The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more like...