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Related Experiment Video

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Real-Time In Vivo Cellular-Level Imaging During Puncture.

Huifang Gao1, Jiakang Shao2,3, Quanzhi Li4,5,6

  • 1College of Optical and Electronic Technology, China Jiliang University, Hangzhou, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|January 5, 2026
PubMed
Summary
This summary is machine-generated.

A novel artificial-intelligence-empowered integrative-light-field microendoscopy (AIM) needle provides real-time, cellular-resolution imaging during biopsy. This technology enhances diagnostic accuracy and reduces sampling risks for improved early disease detection.

Keywords:
biopsy guidancecellular resolutionfalse‐negative rate reductionin vivo puncture imagingmicroscopic visualization

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Area of Science:

  • Biomedical Engineering
  • Medical Imaging
  • Optical Microscopy

Background:

  • Accurate tissue sampling is crucial for disease diagnosis, but current biopsy methods lack real-time cellular visualization.
  • Existing CT/ultrasound-guided biopsies offer macroscopic localization, increasing risks of false-negative or nondiagnostic samples, especially in deep or small targets.

Purpose of the Study:

  • To develop and demonstrate an artificial-intelligence-empowered integrative-light-field microendoscopy (AIM) needle for in vivo, cellular-resolution imaging during biopsy.
  • To overcome limitations of current biopsy techniques by enabling real-time microscopic feedback during puncture.

Main Methods:

  • Co-engineered a photonic-mechanically probe integrating a closed-loop adaptive optics system within a 25G biopsy needle.
  • Utilized light-field modulation and AI (K-means, CNNs) for in situ pathology-like analysis and tissue discrimination.
  • Enabled diffraction-limited imaging during in vivo puncture in mouse models.

Main Results:

  • Successfully resolved characteristic layered microstructures in mouse organs and pulmonary tumors.
  • Demonstrated in situ pathology-like analysis and discrimination between tumor and normal tissues along puncture paths.
  • Achieved diffraction-limited imaging during in vivo puncture, overcoming dynamic diffraction limits.

Conclusions:

  • The AIM needle provides real-time, histology-like feedback during biopsy procedures without interruption.
  • Enhances biopsy targeting accuracy and reduces false-negative rates in challenging cases like narrow lumens and microlesions.
  • Offers potential for improved early disease detection sensitivity through integrated navigation and enhanced sampling.