Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A tunable CTD grammar governs the spatial programming of the transcription cycle.

Nature communications·2026
Same author

CLaSSiNet: A Computational Framework for High-Resolution Classification and Spatial Mapping of Heterogeneous Biological Network Architectures.

JACS Au·2026
Same author

FLEXTAG: a small and self-renewable protein labeling system for anti-fading multi-color super-resolution imaging.

Nature communications·2026
Same author

Actin depolymerization promotes axon regeneration by restoring axonal mitochondrial transport in mouse models of optic neuropathy.

Science translational medicine·2026
Same author

Membrane-associated periodic skeleton regulates major forms of endocytosis in neurons through a signaling-driven positive feedback loop.

Science advances·2026
Same author

Tunable Chemical and Optical Control of ER-Plasma Membrane Contact Site Geometry and Dynamics with High-Fidelity Visualization.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jan 7, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

9.1K

A Chemically Inducible Multimerization System for Tunable and Background-Free RTK Activation.

Yuanmin Zheng1,2, Jinyu Fei1, Abhirup Chakrabarti3

  • 1Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Analytical Chemistry
|January 5, 2026
PubMed
Summary

This study introduces a novel chemically inducible receptor tyrosine kinase (RTK) platform for precise control over cell signaling. The system minimizes unwanted activation, enabling accurate study of ERK pathway dynamics and cell behavior engineering.

More Related Videos

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

13.8K
An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

6.9K

Related Experiment Videos

Last Updated: Jan 7, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

9.1K
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10:09

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

13.8K
An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

6.9K

Area of Science:

  • Cell Biology
  • Molecular Signaling
  • Biotechnology

Background:

  • Receptor tyrosine kinases (RTKs) regulate crucial cellular functions like migration and proliferation.
  • Existing light-inducible RTK systems suffer from high basal activation, causing unintended signaling.
  • Precise spatiotemporal control over RTK signaling is vital for research and engineering applications.

Purpose of the Study:

  • To develop a chemically inducible RTK platform with minimized basal activation.
  • To enable visualization of RTK clustering and downstream signaling.
  • To achieve precise control over ERK-dependent cellular processes.

Main Methods:

  • Development of a chemically inducible RTK system.
  • Single-cell imaging to visualize RTK clustering and ERK phosphorylation.
  • Assessment of effects on spectrin-based membrane skeleton and transcription factor nuclear entry.

Main Results:

  • The new platform significantly minimizes basal RTK activation.
  • Visible RTK clusters and correlated ERK phosphorylation were observed upon induction.
  • Precise control over spectrin disassembly and STAT3/CREB nuclear entry was achieved.
  • The system preserves native cellular architecture and signaling before induction.

Conclusions:

  • The developed platform offers robust and versatile spatiotemporal control over RTK signaling.
  • It enables precise engineering of cell behaviors with on-demand activation.
  • This system overcomes limitations of previous inducible RTK approaches, minimizing unintended effects.