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Updated: May 8, 2026

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
Published on: April 30, 2014
David L Bernholt1, Kurt P Spindler, Fred M Azar
1From the Division Chief of Sports Medicine, Campbell Clinic, University of Tennessee Health Science Center, Germantown, TN (Bernholt and Azar), the Cleveland Clinic Florida, Cleveland, OH (Spindler), Department of Orthopedic Surgery, Vanderbilt University Medical Center, Nashville, TN (Wright).
The two-incision anterior cruciate ligament reconstruction (ACLR) offers clinical benefits for revision surgery and graft selection. This cost-effective technique with metal screws and autograft tendon allows independent femoral tunnel positioning.
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