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Characterisation of a Novel Jumbo Lytic Aeromonas dhakensis Bacteriophage P19 and Its Endolysin

Farhat Ansari1,2, Vandan Nagar1,2

  • 1Food Technology Division, Bhabha Atomic Research Centre, Mumbai, India.

Microbial Biotechnology
|January 6, 2026
PubMed

Insights

Jumbo bacteriophage P19 effectively targets multidrug-resistant Aeromonas dhakensis. Its lytic activity and stable endolysin show promise as novel antimicrobial agents against Gram-negative pathogens.

Area of Science:

  • Microbiology
  • Bacteriophage Therapy
  • Antimicrobial Resistance

Background:

  • Aeromonas dhakensis is an emerging multidrug-resistant (MDR) pathogen threatening public health and aquaculture.
  • Bacteriophage therapy offers a potential alternative to antibiotics for combating MDR bacteria.

Purpose of the Study:

  • To isolate and characterize jumbo bacteriophage P19 for its lytic activity against Aeromonas dhakensis.
  • To evaluate the potential of phage P19 and its endolysin P19_358 as antimicrobial agents.

Main Methods:

  • Isolation and characterization of jumbo bacteriophage P19.
  • Genomic analysis of P19 and identification of endolysin ORF_358.
  • Assessment of lytic activity, stability, and binding affinity of P19 and P19_358.
  • Evaluation of recombinant P19_358 endolysin activity against Gram-positive and Gram-negative bacteria.

Main Results:

  • Phage P19 demonstrated strong lytic activity against A. dhakensis strains, with high stability across a pH range of 6-10 and temperatures up to 60°C.
  • The P19 genome (228 kb) lacks genes for lysogeny, antibiotic resistance, or virulence.
  • Recombinant endolysin P19_358 showed potent lytic activity against Bacillus subtilis and broad-spectrum activity against Gram-negative pathogens, including A. dhakensis.

Conclusions:

  • Phage P19 and its endolysin P19_358 possess significant lytic potential and favorable stability.
  • These findings support the development of P19 and P19_358 as promising antimicrobial agents against MDR Gram-negative pathogens.
Keywords:
Aeromonas dhakensisantibacterial activitybacteriophageendolysingram‐negative bacteria

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