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Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
Published on: December 17, 2011
Peptidoglycan from Bifidobacterium bifidum G9-1 induces IgA secretion in Peyer's patch-derived cells
Hiroaki Konishi1, Koji Yamamoto2, Aki Sakatani3
1Department of Gastroenterology and Advanced Medical Sciences, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510, Japan.
Abstract:
Immunoglobulin A (IgA) plays a key role in mucosal immunity. We investigated the IgA-inducing capacity of heat-killed Bifidobacterium bifidum G9-1 (BBG9-1) using Peyer's patch (PP)-derived cells. Heat-killed BBG9-1, but not its culture supernatant, significantly enhanced IgA secretion and cell aggregation. Compared with other Gram-positive bacteria, BBG9-1 showed a stronger IgA-inducing effect. Component analysis revealed that peptidoglycan (PG), but not lipoteichoic acid (LTA), isolated from BBG9-1 significantly stimulated IgA secretion. These findings suggest that PG from BBG9-1 is a key factor in IgA induction, supporting its potential application in modulating mucosal immune responses.
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