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Fluorescent Staining Using Blankophor for the Diagnosis of Sporotrichosis on Fresh Biopsies
Regielly Caroline Raimundo Cognialli1,2, Marisol Dominguez Muro1, Vânia Aparecida Vicente3
1Hospital de Clínicas, Federal University of Paraná, Curitiba, Brazil.
Abstract:
Sporotrichosis is an implantation mycosis with a high incidence in Brazil. Diagnosing human sporotrichosis poses significant challenges, which can lead to increased morbidity and prolonged treatment duration. Direct examination of fresh biopsies using Blankophor represents a valuable tool for rapid diagnosis, offering high sensitivity.
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The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

