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Updated: Jan 13, 2026
Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA
Published on: February 12, 2020
FAK transfection alleviates myogenic differentiation of C2C12 myoblasts under spike protein intervention: protein
Tianqi Zhang1,2, Hui Peng1,2, Tingting Lu1,2
1Key Laboratory of Metabolic Diseases, Guangxi Zhuang Autonomous Region, Baise, China.
Abstract:
Patients with COVID-19 caused by the novel coronavirus (SARS-CoV-2) often present with long-term muscle weakness symptoms, which are closely related to metabolic disorders. Spike protein (SP) is the main structural protein of SARS-CoV-2, which can upregulate inflammatory factors and affect cell function. This study aims to explore the effect of spike protein on myogenic differentiation of C2C12 myoblasts and its mechanism of action through protein metabolism analysis. The spike protein significantly inhibits the proliferation and differentiation of C2C12 myoblasts, manifested as decreased cell density. Protein metabolism analysis revealed that after spike protein intervention, the pathways related to protein synthesis within cells were inhibited, while those related to protein degradation were activated, indicating that protein metabolism disorders might be an important mechanism of cell damage. FAK transfection can significantly improve cell damage caused by spike proteins, promote myogenic differentiation, and partially restore the normal expression of protein metabolic pathways.
