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Published on: June 25, 2020
Brassinosteroids and TaGSK3 coordinate phosphorus uptake and low-phosphorus tolerance in wheat through overlapping
Pengcheng Wang1, Jialiang Zhang1, Chen Yang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
Abstract:
Brassinosteroids (BRs) regulate plant growth and stress responses, but their role in wheat's phosphate (Pi) uptake and low-phosphorus (LP) tolerance is unclear. This study reveals that BR signaling enhances Pi uptake and LP tolerance by modulating the expression of key genes involved in Pi uptake, transport and LP response. Consistently, the sextuple mutants of TaGSK3, encoding the key repressor of the BR signaling, increased Pi uptake by 16.42 %. Unexpectedly, the TaGSK3 gain-of-function mutants also exhibited a 129.90-202.30 % increase in Pi uptake, mediated by their increased kinase activity. Under LP stress, sextuple mutants of TaGSK3 exhibited enhanced tillering and root elongation, whereas TaGSK3 gain-of-function mutants increased root biomass, suggesting the functional state of TaGSK3 affects the trade-off strategies for above-ground and below-ground growth. Overall, these results uncover a complex regulatory network in which BR signaling and TaGSK3 collaboratively and independently coordinate Pi homeostasis, offering novel targets for improving phosphorus use efficiency in wheat.
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