Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Surface Membrane Barriers

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Resolution of childhood wheat allergy through evolution into WALDA? A case report.

Allergologie select·2026

Same author

Demonstration of Gliadin Penetration into the Epidermis by Tape Stripping as Prerequisite for Percutaneous Sensitisation in Wheat Allergy.

International journal of molecular sciences·2026

Same author

Addictions in Hidradenitis Suppurativa: A Cross-Sectional Pilot Study.

Dermatology and therapy·2026

Same author

Hypersensitivity to Excipients in Drugs: An EAACI Position Paper.

Allergy·2026

Same author

Casein as rare elicitor of food allergy dependent on augmentation factors - A case report.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2026

Same author

Rare follicular variant of lichen planus pigmentosus and concomitant demodicosis with excellent treatment response.

Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·2026

Search research articles

Related Experiment Video

Updated: May 5, 2026

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Published on: March 28, 2014

Assessing Skin Barrier Integrity: A Comparative Study Using Transepidermal Water Loss, Electrical Impedance

Charlotte Jasmin Kiani¹, Valentina Faihs¹, Claudia Kugler¹

¹Department of Dermatology and Allergy Biederstein, School of Medicine and Health, TUM University Hospital Rechts der Isar, Munich, Germany.

Contact Dermatitis

|January 6, 2026

Summary

This summary is machine-generated.

Electrical impedance spectroscopy (EIS) shows strong agreement with established methods for measuring skin barrier disruption. Combined mechanical and chemical stress provides a potent model for evaluating skin barrier impairment in vivo.

Keywords:

allergen penetration corneometry electrical impedance spectroscopy skin barrier transepidermal water loss

More Related Videos

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Published on: March 1, 2017

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Published on: September 26, 2025

Related Experiment Videos

Last Updated: May 5, 2026

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Published on: March 28, 2014

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Published on: March 1, 2017

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Development, Characterization, and Evaluation of CAGE-based Ionic Liquid Systems for Transdermal Delivery

Published on: September 26, 2025

Area of Science:

Dermatology
Biophysics
Skin Physiology

Background:

Standardized methods for assessing experimental skin barrier disruption are limited.
Transepidermal water loss (TEWL) and corneometry (CM) are established but novel methods like electrical impedance spectroscopy (EIS) may offer complementary insights.

Purpose of the Study:

To compare the efficacy of EIS against TEWL and CM in quantifying skin barrier impairment.
To evaluate various experimental models of skin barrier disruption in human subjects in vivo.

Main Methods:

15 healthy adults participated in the study.
Experiment I: Compared three adhesive tapes for tape stripping (TS).
Experiment II: Assessed TS, sodium lauryl sulphate (SLS), SLS + TS, and gluten using TEWL, EIS, and CM.

Main Results:

D-Squame tape induced greater barrier impairment than Scotch or Tesa tape.
SLS + TS caused the most significant and persistent disruption, followed by TS alone.
EIS demonstrated strong correlations with both TEWL (ρ = -0.62) and CM (ρ = -0.61).

Conclusions:

This study is the first to demonstrate the strong concordance of EIS with TEWL and CM in human subjects following experimental barrier disruption.
Combined mechanical and chemical stress represents a potent in vivo model for skin barrier insults, closely mimicking real-life conditions.