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Related Concept Videos

Hydrolysis01:15

Hydrolysis

121.0K
Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
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Lysosomal Hydrolases01:22

Lysosomal Hydrolases

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Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
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Related Experiment Video

Updated: Jan 13, 2026

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
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Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Published on: October 30, 2013

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Precision hydrolysis: tailored yeast processing enzymes for yeast-based products.

Jieying Deng1,2, Zhendong Li3, Xueqin Lv1,2

  • 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.

Applied Microbiology and Biotechnology
|January 6, 2026
PubMed
Summary

Precision hydrolysis uses specific enzymes to precisely extract valuable components from yeast, improving product quality for food, feed, and medicine. Advances in enzyme technology are crucial for cost-effective, sustainable yeast bioproduct development.

Keywords:
Flavor enzymesNucleasesPrecision hydrolysisProteasesYeast derivatesYeast processing enzymesβ-Glucanases

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High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
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High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

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Area of Science:

  • Biotechnology
  • Enzymology
  • Food Science

Background:

  • Yeasts are versatile bioresources utilized in food, feed, medicine, and cosmetics.
  • Traditional yeast processing methods often lead to non-specific lysis and reduced product quality.
  • Yeast components like extract, glucans, and nucleotides possess significant nutritional and therapeutic value.

Purpose of the Study:

  • To review the mechanistic properties and applications of yeast processing enzymes in precision hydrolysis.
  • To highlight strategies for optimizing enzyme biosynthesis for high-value yeast-based bioproducts.
  • To support innovation in sustainable and diversified yeast bioproduct development.

Main Methods:

  • Focus on mechanistically specific enzymes such as proteases, β-glucanases, and nucleases-deaminases.
  • Detailed review of enzyme classification and their role in targeted component release.
  • Exploration of enzyme discovery, heterologous expression, and machine learning for enzyme optimization.

Main Results:

  • Precision hydrolysis offers superior yield and functional quality of yeast-based products compared to traditional methods.
  • Tailored enzymatic strategies enhance nutritional value and sensory profiles of yeast products.
  • Optimized enzyme biosynthesis can lower the cost of precision hydrolysis.

Conclusions:

  • Precision hydrolysis enables controlled yeast component release for high-quality products.
  • Enzymatic specificity allows for targeted refinement under mild conditions.
  • Continuous advancements in enzyme engineering and discovery are vital for cost-effective precision hydrolysis.