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Updated: Jan 13, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Argonaute-mediated RNA editing selectively repairs point mutations.

Zhiwei Zhang¹, Jinyue Wang¹, Tongyu Guo¹

¹State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, Hubei 430062, China.

Nucleic Acids Research

|January 7, 2026

Summary

This study introduces McAgo, a programmable nuclease for RNA knockdown and editing in mammalian cells. It offers precise gene expression control without altering DNA, expanding Argonaute protein functions.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

Molecular Biology
Gene Regulation
Biotechnology

Background:

RNA editing offers dynamic gene regulation without permanent genomic changes.
Argonaute proteins are key in RNA interference pathways.

Purpose of the Study:

To characterize McAgo from Monosporascus cannonballus for RNA targeting.
To evaluate McAgo's potential for RNA knockdown and editing in mammalian cells.

Main Methods:

Characterization of McAgo nuclease activity guided by small RNAs.
Delivery of McAgo ribonucleoprotein (RNP) complexes into mammalian cells.
In vitro RNA editing assays using a catalytically inactive McAgo mutant conjugated to hADAR2 deaminase.

Main Results:

McAgo demonstrated robust RNA cleavage activity at physiological temperatures.
McAgo RNP complexes achieved >90% endogenous RNA knockdown in mammalian cells with low immune response.
A dMcAgo-hADAR2dd conjugate achieved up to 90% RNA editing efficiency in vitro.

Conclusions:

McAgo effectively targets endogenous RNA in mammalian cells.
This work expands the utility of Argonaute proteins for RNA knockdown and editing.
The findings pave the way for new RNA-based therapeutic strategies.