Specificity of the stabilizing interaction between intrinsically disordered protein sequences and G-quadruplexes in...

Intrinsically disordered regions (IDRs) are present in and essential for the function of nearly all the proteins involved in regulation, cell, and developmental processes. The RGG domain in IDRs binds 'promiscuously' to RNA G-quadruplexes (rG4s), a non-canonical 4-stranded secondary structure that occurs in many transcripts involved in gene regulation. Here we show, using weak binding interactions between a minimal RGG-rich peptide and rG4s, that the IDR selectively templates and stabilizes the structure of the human telomeric TERRA rG4, providing a unique pathway to RNA folding that does not rely on high-affinity binding or monovalent cations. Multidimensional NMR and circular dichroism (CD) spectroscopy analyses reveal individual nucleotide and amino acid identities determine the specificity of the interaction between RGG peptides and rG4s, explaining how IDRs can selectively recognize RNA over DNA G4s, the high specificity of such interactions in vivo, and the high frequency of monogenic mutations observed in IDRs.