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Response letter to Rebuttal to Zimmermann et al. (2025) critique of TerL-based PCR

Manja Zimmermann1, Gabriele Margos1, Christine Hartberger1

  • 1Bavarian Health and Food Safety Authority, German National Reference Center for Borrelia, Veterinärstraße 2, 85764 Oberschleissheim, Germany.

Ticks and Tick-Borne Diseases
|January 7, 2026
PubMed
Abstract

No abstract available in PubMed .

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PCR01:32

PCR

Overview
Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...