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LncRNA PCED1B-AS1 Inhibits Sepsis-Induced Acute Kidney Injury by Promoting Transformation of Macrophages from M1 to

Peng Huang1,2, Youfu Li3, SiDan Wang1

  • 1Department of Nephrology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi, People's Republic of China.

Journal of Inflammation Research
|January 7, 2026
PubMed
Abstract

Insights

Long non-coding RNA PCED1B-AS1 is downregulated in sepsis-induced acute kidney injury (AKI). Upregulating PCED1B-AS1 may inhibit AKI by promoting M2 macrophage polarization via the miR-361-3p/SOCS1 axis.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Immunology

Background:

  • Sepsis-induced acute kidney injury (AKI) is a critical complication in intensive care with poor outcomes.
  • Understanding the molecular mechanisms underlying sepsis-AKI is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the expression of PCED1B-AS1 in sepsis-induced AKI.
  • To explore the potential molecular mechanism of PCED1B-AS1 in sepsis-AKI.

Main Methods:

  • RT-qPCR to quantify PCED1B-AS1 levels in patients and cell models.
  • In vitro models using RAW264.7 and HK-2 cells to study macrophage polarization and renal injury.
  • CCK-8, flow cytometry, and ELISA to assess cell viability, apoptosis, and inflammatory factors.
  • Dual-luciferase assay to validate the PCED1B-AS1/miR-361-3p/SOCS1 axis interaction.

Main Results:

  • PCED1B-AS1 expression was significantly downregulated in sepsis patients, with further reduction in AKI patients.
  • PCED1B-AS1 levels could distinguish healthy individuals, non-AKI sepsis patients, and AKI patients, correlating with prognosis.
  • Overexpression of PCED1B-AS1 promoted M1 to M2 macrophage polarization, inhibited HK-2 cell apoptosis, and reduced inflammation via the miR-361-3p/SOCS1 pathway.

Conclusions:

  • PCED1B-AS1 may serve as a valuable diagnostic and prognostic biomarker for sepsis-induced AKI.
  • PCED1B-AS1 inhibits sepsis-induced AKI by modulating macrophage polarization and inflammatory responses through the miR-361-3p/SOCS1 axis.
Keywords:
PCED1B-AS1diagnosismacrophage polarizationmiR-361-3pprognosis

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