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Updated: Jan 13, 2026
07:58
Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
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Vibrio cholerae serotype impacts pathogenicity
Franz G Zingl1,2,3, Deborah R Leitner1,2,3, Bolutife Fakoya1,2,3
1Division of Infectious Diseases, Brigham & Women's Hospital, Boston, MA.
Biorxiv : the Preprint Server for Biology
|January 7, 2026
Summary
Methylation of the Vibrio cholerae O1 antigen in Ogawa strains enhances bacterial colonization and infectivity. This O1 antigen methylation shields the bacterium from antimicrobial peptides, aiding survival in the small intestine.
Area of Science:
- Microbiology
- Immunology
- Molecular Biology
Background:
- Vibrio cholerae O1 causes cholera pandemics.
- V. cholerae O1 strains are characterized by serotypes Ogawa and Inaba.
- Serotype switching is linked to pathogen adaptation and immune pressure.
Purpose of the Study:
- To investigate the impact of O1 antigen methylation on V. cholerae pathogenicity.
- To compare isogenic Ogawa and Inaba V. cholerae O1 strains.
- To elucidate the role of serotype in V. cholerae colonization and infectivity.
Main Methods:
- Utilized isogenic Ogawa and Inaba V. cholerae O1 clinical isolates.
- Assessed bacterial colonization, infectivity, and resistance to antimicrobial peptides.
- Examined the effect of O1 antigen methylation on V. cholerae survival.
Main Results:
- O1 antigen methylation in Ogawa strains significantly promotes V. cholerae colonization.
- Ogawa strains exhibit increased infectivity compared to Inaba strains.
- Methylation confers resistance to cationic antimicrobial peptides.
Conclusions:
- O1 antigen methylation is crucial for V. cholerae colonization and infectivity.
- Methylation shields V. cholerae from antimicrobial peptides in the small intestine.
- These findings highlight the biological significance of V. cholerae O1 serotypes.
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