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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
Published on: September 25, 2017
Pectin-modified PCN-222 for detecting nitrofurazone residues with dual signals in livestock, poultry, and aquatic
Keyu Du1,2, Jie Shen1,2, Siyao Zhong1,2
1Key Laboratory of Microbiological Metrology, Measurement & Bio-product Quality Security, State Administration for Market Regulation, College of Life Science, China Jiliang University, Hangzhou 310018, China.
Abstract:
Nitrofurazone and its metabolite semicarbazide (SEM) residues present in food products pose substantial health hazards to humans. Herein, the colorimetric-fluorescent lateral flow immunoassay (LFIA) for 4[(4-carboxyphenyl)-methylene]-hydrazinecarboxamide (CPSEM) detection was developed using PCN-222@Pectin as a signal tag. Herein, the PCN-222 surface was subsequently modified with pectin to enhance its aqueous solubility and stability. The PCN-222@Pectin-LFIA system exhibited a broad linear range from 0.025 ng/mL to 100 ng/mL. The low limits of detection for the colorimetric and fluorescence-based detection methods were 0.1 and 0.05 ng/mL, respectively. Notably, these values represent a 10- and 20-fold higher sensitivity than that of the traditional colloidal gold nanoparticle-based LFIA. This method was successfully used to detect CPSEM in actual samples, with recovery rates of 89.34 %-120.80 %. This approach fulfills the need for on-site preliminary qualitative assessment during detection and permits subsequent accurate quantitative analysis.
