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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

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Expanding the Toolbox for Inducible Protein Expression With Automation-enabled Generation of Glycomimetics.

Ashley E DeYong¹, Keevan C Marion¹, Murat Ozturk¹

¹Chemistry, Indiana University, Bloomington, Indiana, USA.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|January 7, 2026

Summary

Scientists developed a novel inducer molecule that controls protein expression in biotechnological processes. This fucose-containing compound enables gene expression only in specific cells, offering enhanced control for industrial applications.

Keywords:

automation carbohydrates continuous flow protein expression synthetic biology

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Area of Science:

Biotechnology
Molecular Biology
Synthetic Chemistry

Background:

Inducible protein expression is crucial for industrial and molecular biotechnology.
Limited inducible transcription factors restrict population-level control of gene expression.

Purpose of the Study:

To design and synthesize a novel inducer for enhanced control of protein expression.
To develop a fucose-containing molecule that triggers gene expression via the lac operon in a cell-specific manner.

Main Methods:

Synthesis of 2'-fucosyl isopropyl-β-D-thiogalactopyranose (2'F-IPTG) and isobutyl-C-galactoside (2'F-IBCG) mimics using batch and continuous-flow processes.
Demonstration of fucosidase-dependent reporter protein expression.
Utilizing the lac operon for inducible gene expression.

Main Results:

Successful synthesis of novel fucose-containing inducers (2'F-IPTG and 2'F-IBCG).
Achieved fucosidase-dependent induction of protein expression.
Demonstrated cell-specific control over gene expression through selective de-fucosylation.

Conclusions:

The novel fucose-modified inducers provide an additional layer of control for inducible gene expression.
This approach enhances the precision of controlling protein expression in biotechnological systems.
The developed synthetic methods are applicable to both batch and continuous-flow processes.