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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
Published on: June 27, 2017
Expanding the Toolbox for Inducible Protein Expression With Automation-enabled Generation of Glycomimetics
Ashley E DeYong1, Keevan C Marion1, Murat Ozturk1
1Chemistry, Indiana University, Bloomington, Indiana, USA.
Abstract:
Inducible protein expression is a cornerstone of many aspects of industrial and molecular biotechnological processes. However, limited availability of inducible transcription factors can reduce our ability to control expression at a population level. The design and synthesis of a powerful inducer containing a fucose is demonstrated to induce protein expression through the lac operon only in cells with the ability to selectively de-fucosylate them. Batch and automated continuous-flow processes are reported for the syntheses of both 2'-fucosyl isopropyl-β-D-thiogalactopyranose (2'F-IPTG) and isobutyl-C-galactoside (2'F-IBCG) mimics. Fucosylation of the inducer allowed for fucosidase-dependent expression of a reporter protein, providing an additional layer of control over inducible gene expression.
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