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Related Concept Videos

Caspases01:24

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.

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In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
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Fouling-Proof Nanopore for Detecting Caspase-3 in Complex Biological Fluids.

Shujie Zhang1, Ruina Zheng1, Weiling Yu1

  • 1Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, P. R. China.

Analytical Chemistry
|January 7, 2026
PubMed
Summary

This study developed an antifouling nanopore biosensor for detecting Caspase-3, a key apoptosis marker. The novel biosensor effectively detects Caspase-3 protease in complex biological samples with high sensitivity and selectivity.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Biochemistry

Background:

  • Caspase-3 is a critical marker for apoptosis and related diseases.
  • Detecting Caspase-3 in complex biological samples is challenging due to interference.

Purpose of the Study:

  • To develop an antifouling nanopore biosensor for accurate Caspase-3 detection.
  • To overcome interference issues in biological sample analysis.

Main Methods:

  • Constructed a glass nanopore biosensor integrating DSPE-PEG and a Caspase-3-targeted peptide.
  • Characterized antifouling properties using contact angle, electrochemical, and confocal imaging.
  • Sensed Caspase-3 by monitoring changes in nanopore rectified ionic current.

Main Results:

  • The biosensor demonstrated excellent antifouling capabilities.
  • Achieved a sensitive detection limit of 0.46 ng/mL for Caspase-3.
  • Exhibited high selectivity against interfering proteins in biological environments.

Conclusions:

  • The antifouling nanopore biosensor provides a robust platform for Caspase-3 detection.
  • This technology facilitates Caspase-3 protease analysis in complex biological settings.
  • The biosensor shows promise for apoptosis-related disease diagnosis and therapy.