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Related Experiment Video

Updated: Jan 13, 2026

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Protocol for developing a mucus-producing gut-on-a-chip model from Caco-2 and HT29-MTX-E12 cells.

Valerija Movcana1, Vendija Kozlova2, Arnis Strods3

  • 1Cellbox Labs LTD, 2146 Atari, Latvia.

STAR Protocols
|January 7, 2026
PubMed
Summary

Organ-on-chip technology creates advanced gut models that mimic in vivo conditions. This study details a protocol for a mucus-producing gut-on-a-chip model using Caco-2 and HT29-MTX-E12 cells.

Keywords:
biotechnology and bioengineeringcell biologytissue engineering

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Gastroenterology

Background:

  • Static in vitro gut models lack physiological relevance.
  • Organ-on-chip (OoC) technology offers improved simulation of in vivo conditions.
  • Replication of 3D structure, mucus production, fluid dynamics, and mechanical forces is crucial for accurate gut modeling.

Purpose of the Study:

  • To present a protocol for developing a mucus-producing gut-on-a-chip model.
  • To utilize Caco-2 and HT29-MTX-E12 cells for constructing the gut-on-a-chip system.
  • To establish methods for assessing the model's viability and barrier integrity.

Main Methods:

  • Development of a gut-on-a-chip model using Caco-2 and HT29-MTX-E12 cells.
  • Assessment of model viability and barrier integrity.
  • Application of various microscopy techniques, including confocal microscopy, for analysis.

Main Results:

  • Successful development of a mucus-producing gut-on-a-chip model.
  • Demonstrated methods for evaluating the model's physiological relevance and functionality.
  • Characterization of the model using advanced imaging techniques.

Conclusions:

  • The developed protocol enables the creation of a physiologically relevant gut-on-a-chip model.
  • This OoC model closely mimics in vivo gut conditions, including mucus production.
  • The protocol provides a valuable tool for studying gut physiology and disease in vitro.