Functional promotion of uranium bioremediation in recombinant Shewanella azerbaijanica

Bioremediation is a sustainable Biological strategy, to remove hazardous substances from the contaminated areas by living organisms. Shewanella azerbaijanica is introduced as a highly potent bacterium in metal bioremediation. The current study has applied a comparison of functional bioremediation processes in recombinant (RSh) and native (NSh) Shewanella azerbaijanica bacteria. The RSh maintained the cloned mtrC gene as a principal gene in the EET (Extracellular Electron Transfer) pathway. The uranium bioremediation procedure evaluated through cell counting and ICP analysis in RSh and NSh, Despite, fewer viable cells, RSh showed 21 % higher uranium removal efficiency than NSh, likely due to the metabolic load of plasmid maintenance and recombinant protein expression enhancing electron transfer for uranium reduction. The uranium reduction process was confirmed through XRD and UV-Vis spectroscopy in RSh. Thereupon, the recombinant bacteria have been proposed as an efficient genetically manipulated strain in future metals bioremediation studies.