Correlation Study of LncRNA H19 Single Nucleotide Polymorphism With Delayed Graft Function of Transplanted Kidney: A...

Area of Science:

• Genetics
• Transplantation immunology
• Molecular biology

Background:

• Delayed graft function (DGF) is a common complication after kidney transplantation, impacting graft survival.
• Long non-coding RNAs (lncRNAs) play roles in cellular processes, and their genetic variations may influence transplant outcomes.
• LncRNA H19 has been implicated in various biological functions, but its role in DGF requires further investigation.

Purpose of the Study:

• To investigate the association between specific single nucleotide polymorphisms (SNPs) in LncRNA H19 and the occurrence of DGF after kidney transplantation.
• To identify potential genetic markers for predicting DGF risk.

Main Methods:

• A retrospective study of 827 kidney transplantations was conducted.
• Fifty recipients with DGF and fifty with immediate graft function (IGF) were selected, with donor kidneys matched between groups.
• Genotyping of LncRNA H19 SNPs (rs217727, rs2067051, rs2251375, rs492994, rs2839698, rs10732516) was performed using peripheral blood samples.

Main Results:

• Significant differences in SNP frequencies were observed between DGF and IGF groups at the rs492994 and rs2839698 loci (P < .05).
• No statistically significant differences in SNP frequencies were found at the other four LncRNA H19 loci (P > .05).
• Donor and recipient characteristics, as well as transplant-related factors, were comparable between the DGF and IGF groups, ruling out confounding factors.

Conclusions:

• The LncRNA H19 polymorphisms rs492994 and rs2839698 are significantly associated with the occurrence of DGF following kidney transplantation.
• These specific SNPs may serve as potential predictive biomarkers for DGF.