Related Experiment Video
Updated: Jan 13, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete genome sequence of a novel mitovirus isolated from the fungus Phomopsis asparagi
Yufang Zhang1,2, Zhibiao Wang1,2, Jinyu Yang1,2
1National Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, Hainan, China.
Abstract:
Here, we report the isolation of a novel mycovirus from Phomopsis asparagi strain XJ5. The viral genome consists of a positive-sense, single-stranded RNA (+ ssRNA) molecule that is 2,657 nucleotides (nt) in length and has a GC content of 42%. It contains a single open reading frame (ORF) encoding a putative protein of 731 amino acids, with a predicted molecular mass of 83.0 kDa. A BLASTp search showed that the putative protein shared the highest sequence similarity with Fusarium pseudograminearum mitovirus 5 (FpgMV5), exhibiting 55.84% sequence identity. Phylogenetic analysis revealed that this mycovirus clusters with members of the genus Unuamitovirus, belonging to the family Mitoviridae. Based on these findings, we propose to designate this virus as "Phomopsis asparagi mitovirus 1" (PhaMV1).
Related Concept Videos
Fungal Phylum Microsporidia
Fungal Phylum Ascomycota
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Viruses of Archaea

