Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Type IV Collagen of Basal Lamina

Type IV Collagen of Basal Lamina

Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can exist in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

P-Selectin and Platelet-Monocyte Interaction in Inflammation: Mechanisms, Hypothesis, and Open Questions.

Journal of inflammation research·2026

Same author

Chitosan Films Incorporating Cellulose Nanofibers or Carbon Nanotubes Differentially Modulate Early Responses of Stem Cells from Human Exfoliated Deciduous Teeth.

ACS applied bio materials·2026

Same author

Innate Immunity Trained in the Protective Response of Vaccine Candidates Against Intracellular Pathogens.

Vaccines·2026

Same author

Reply to "comment on: Vegetable peptones as a fetal bovine serum substitute in human deciduous tooth pulp stem cell culture".

Einstein (Sao Paulo, Brazil)·2026

Same author

A new epoxy for plastination: feasibility and applicability analysis of the conservation of biological tissues.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica·2025

Same author

Structural and Viability Assessment of Bovine Corneas Preserved at an Accessible Low-Temperature (-20 °C) for Eye Irritation Models.

ACS omega·2025

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Hydrogel Based on Decellularized Bovine Trabecular Extracellular Matrix Enriched with Type I Collagen.

Marizia Trevizani¹, Laís Lopardi Leal¹, Gabriela Coelho Floriano¹

¹Department of Biology, Laboratory of Human Genetics and Cell Therapy, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora 36036-900, Minas Gerais, Brazil.

|January 8, 2026

Summary

This study developed a novel thermosensitive biocomposite hydrogel from bone extracellular matrix (ECM) and tendon collagen. This biomaterial shows promise for tissue engineering and regenerative medicine, particularly in bone repair applications.

More Related Videos

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Published on: July 21, 2023

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Published on: December 8, 2023

Related Experiment Videos

Last Updated: Jun 3, 2026

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Synthesis of Decellularized Cartilage Extracellular Matrix Hydrogels

Published on: July 21, 2023

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Published on: December 8, 2023

Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

Biomaterials are crucial for tissue replacement, requiring biomimetic, biocompatible, and biodegradable properties.
Biocomposites offer enhanced chemical and mechanical characteristics for biological integration and stability at implantation sites.
Developing advanced biomaterials is essential for addressing unmet needs in tissue repair and regeneration.

Purpose of the Study:

To develop a thermosensitive biocomposite hydrogel using decellularized extracellular matrix (ECM) from bovine trabecular bone and type I collagen from bovine tendon.
To evaluate the incorporation of ECM components and assess the hydrogel's thermosensitive properties.
To investigate the potential of this novel biocomposite for tissue engineering applications, specifically in bone repair.

Main Methods:

Bovine bone fragments were decellularized, lyophilized, and pulverized to obtain ECM.
Type I collagen was extracted from bovine tendons.
The ECM and tendon collagen were combined, digested, neutralized, and incubated at 37°C to induce gelation, with characterization via turbidimetry and SEM.

Main Results:

The developed hydrogel exhibited thermosensitivity, with gelation completed in approximately 50 minutes.
High incorporation rates were achieved for collagen (96.7%) and glycosaminoglycans (GAGs) (109%).
Scanning electron microscopy revealed a porous, reticulated structure, indicative of a suitable scaffold for tissue regeneration.

Conclusions:

A thermosensitive biocomposite hydrogel incorporating bone ECM and tendon collagen was successfully developed.
The hydrogel demonstrates excellent material properties and efficient incorporation of key biomolecules.
This novel biomaterial holds significant potential for applications in tissue engineering and regenerative medicine, particularly for bone defect repair.