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Hydrogel Based on Decellularized Bovine Trabecular Extracellular Matrix Enriched with Type I Collagen
Marizia Trevizani1, Laís Lopardi Leal1, Gabriela Coelho Floriano1
1Department of Biology, Laboratory of Human Genetics and Cell Therapy, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora 36036-900, Minas Gerais, Brazil.
ACS Omega
|January 8, 2026
Summary
This study developed a novel thermosensitive biocomposite hydrogel from bone extracellular matrix (ECM) and tendon collagen. This biomaterial shows promise for tissue engineering and regenerative medicine, particularly in bone repair applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Biomaterials are crucial for tissue replacement, requiring biomimetic, biocompatible, and biodegradable properties.
- Biocomposites offer enhanced chemical and mechanical characteristics for biological integration and stability at implantation sites.
- Developing advanced biomaterials is essential for addressing unmet needs in tissue repair and regeneration.
Purpose of the Study:
- To develop a thermosensitive biocomposite hydrogel using decellularized extracellular matrix (ECM) from bovine trabecular bone and type I collagen from bovine tendon.
- To evaluate the incorporation of ECM components and assess the hydrogel's thermosensitive properties.
- To investigate the potential of this novel biocomposite for tissue engineering applications, specifically in bone repair.
Main Methods:
- Bovine bone fragments were decellularized, lyophilized, and pulverized to obtain ECM.
- Type I collagen was extracted from bovine tendons.
- The ECM and tendon collagen were combined, digested, neutralized, and incubated at 37°C to induce gelation, with characterization via turbidimetry and SEM.
Main Results:
- The developed hydrogel exhibited thermosensitivity, with gelation completed in approximately 50 minutes.
- High incorporation rates were achieved for collagen (96.7%) and glycosaminoglycans (GAGs) (109%).
- Scanning electron microscopy revealed a porous, reticulated structure, indicative of a suitable scaffold for tissue regeneration.
Conclusions:
- A thermosensitive biocomposite hydrogel incorporating bone ECM and tendon collagen was successfully developed.
- The hydrogel demonstrates excellent material properties and efficient incorporation of key biomolecules.
- This novel biomaterial holds significant potential for applications in tissue engineering and regenerative medicine, particularly for bone defect repair.
