Related Experiment Video
Updated: Jun 23, 2026
08:18
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Published on: May 12, 2023
2.6K
Integrative Phytochemical and Transcriptomic Analysis Reveals Genes Regulating 14-Deoxyandrographolide in Thai
Neeranuch Thongkan1, Worathat Thitikornpong2, Amorntip Muangprom3
1Center of Excellence in DNA Barcoding of Thai Medicinal Plants, Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
ACS Omega
|January 8, 2026
Summary
This study investigated genetic factors influencing 14-deoxyandrographolide (AP6) in Andrographis paniculata. It identified candidate genes related to AP6 biosynthesis, aiding in standardizing this medicinal plant's quality.
Area of Science:
- Phytochemistry
- Molecular Biology
- Genetics
Background:
- Andrographis paniculata is a medicinal herb valued for bioactive diterpenoid lactones like andrographolide (AP1).
- Regional cultivation leads to significant variability in phytochemical profiles, impacting raw material quality and therapeutic consistency.
- 14-deoxyandrographolide (AP6) content exhibits notable variation, suggesting a genetic basis for its accumulation.
Purpose of the Study:
- To comprehensively profile diterpenoid lactones and flavonoids in 40 Thai Andrographis paniculata accessions.
- To identify accessions with contrasting AP6 content for further investigation.
- To gain preliminary insights into candidate genes involved in AP6 biosynthesis using comparative transcriptomics.
Main Methods:
- Phytochemical profiling using HPTLC and HPLC to quantify diterpenoid lactones (AP1, AP2, AP4, AP6).
- UPGMA cluster analysis to classify accessions into high- and low-AP6 groups.
- RNA sequencing (RNA-seq) to identify differentially expressed genes (DEGs) between contrasting AP6 groups.
Main Results:
- Four major diterpenoid lactones were identified, with AP1 being most abundant and AP6 showing greatest variability.
- Comparative transcriptomic analysis revealed 255 DEGs between high- and low-AP6 groups.
- Candidate genes, including CYP94C1-like, CYP84A1-like, and beta-amyrin 28-monooxygenase-like, were identified as potentially involved in AP6 biosynthesis.
Conclusions:
- This study integrates phytochemical and transcriptomic data to explore the genetic regulation of AP6 accumulation in Andrographis paniculata.
- Novel candidate genes for AP6 biosynthesis were identified, providing a basis for future functional validation.
- Findings support phytochemical standardization, precise cultivar selection, and development of value-added herbal products from Andrographis paniculata.
