AQP9 in Neutrophils Promotes the Formation of NETs by Regulating JAK2-STAT3 Pathway-Mediated Pyroptosis to Aggravate...

Ulcerative colitis (UC) is a chronic inflammatory disorder primarily affecting the rectum and colon. This study aimed to identify potential therapeutic targets that may inhibit UC progression and mitigate patient suffering.

UC-related datasets were retrieved from the Gene Expression Omnibus database. Differential expression analysis, weighted gene co-expression network analysis, immunoinfiltration analysis, and pyroptosis scoring were employed to identify key pyroptosis-related genes implicated in UC pathogenesis. A dextran sulfate sodium salt (DSS)-induced mouse model of UC was established, and neutrophil extracellular traps (NETs) were induced in neutrophils by stimulation with phorbol 12-myristate 13-acetate (PMA). Histopathological changes in mouse colon tissues were assessed by hematoxylin-eosin staining, and NET formation was evaluated via immunofluorescence. The expression of aquaporin 9 (AQP9), peptidylarginine deiminase 4 (PAD4), zonula occludens 1 (ZO-1), occludin, and proteins related to pyroptosis and the JAK2-STAT3 pathway was determined by Western blotting. Levels of inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA), production of reactive oxygen species was assessed using fluorescent probes, and intestinal epithelial cell viability and death were evaluated using the cell counting kit-8 (CCK-8) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, respectively.

Five hub genes (AQP9, S100A8, S100A9, S100A12, and VNN2) were identified through bioinformatics analysis, with AQP9 selected for further investigation. Single-cell analysis and immunofluorescence revealed that AQP9 was predominantly expressed in neutrophils and upregulated in the colon tissues of mice with UC and PMA-stimulated neutrophils. Knockdown of AQP9 in PMA-treated neutrophils led to suppression of the JAK2-STAT3 pathway, reduced pyroptosis, and decreased NET formation. Upon co-culture with intestinal epithelial cells, AQP9 knockdown resulted in enhanced epithelial cell viability, reduced apoptosis, and upregulation of ZO-1 and occludin. Conversely, treatment of neutrophils from the PMA+si-AQP9 with a JAK2-STAT3 pathway agonist increased pyroptosis, enhanced the formation of NETs, and induced epithelial cell injury. Similarly, treatment with a pyroptosis agonist enhanced both pyroptosis and the formation of NETs, further aggravating epithelial damage.

Knockdown of AQP9 inhibits JAK2-STAT3 pathway-mediated pyroptosis, thereby reducing the formation of NETs and attenuating intestinal epithelial cell injury.