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Ultrasensitive Biosensing Platform Based on Hydroxylamine-Enhanced Copper-Mediated Fenton-Like Reaction: Application
Tingting Ma1, Yiran Liu1, Maoguo Li1
1The Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China.
Analytical Chemistry
|January 8, 2026
Summary
This study introduces a new hydroxylamine-mediated copper system for sensitive detection of copper ions and nucleic acids, like hepatitis B virus DNA, at neutral pH.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Nanotechnology
Background:
- Reactive oxygen species (ROS)-based biosensing is crucial for disease diagnosis.
- Traditional Fenton reactions face limitations due to pH sensitivity and metal ion hydrolysis.
- Hydroxylamine (HA) offers a promising alternative for ROS generation in biosensing.
Purpose of the Study:
- To develop a novel hydroxylamine-mediated copper-based Fenton-like reaction (FLR) system.
- To engineer an efficient amplification mechanism for hydroxyl radical (•OH) generation.
- To create ultrasensitive biosensors for copper ions and nucleic acids, including hepatitis B virus (HBV) DNA.
Main Methods:
- Developed a Cu(II)/HA system for amplified •OH generation at neutral pH.
- Synthesized magnetic carboxylated copper-loaded iron oxide nanoparticles (Cu/Fe3O4-COOH) as nanocarriers.
- Designed a DNA hybridization assay utilizing Cu/Fe3O4-COOH nanoparticles for HBV DNA detection.
Main Results:
- Achieved a 5.6-fold amplification of •OH generation.
- Developed a copper ion detection assay with a low limit of detection (LOD) of 0.01 nM.
- Demonstrated ultrasensitive and sequence-specific detection of HBV DNA with an LOD of 0.06 nM in clinical samples.
Conclusions:
- The HA-mediated Cu-FLR system provides a robust platform for neutral pH biosensing.
- Cu/Fe3O4-COOH nanoparticles serve as efficient nanocarriers for ultrasensitive nucleic acid detection.
- This research advances metal ion sensing and nucleic acid biosensor design, with broad applications for nanomaterials.
