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Quantitative Proteomics Analysis Insights into the Cordyceps Polysaccharides Biosynthesis.
Chunhua Xu1, Yifan Guo2, Xiaoni Chen2
1Department of Nephrology, Longgang District Central Hospital of Shenzhen, Shenzhen, Guangdong, China.
Applied Biochemistry and Biotechnology
|January 8, 2026
Summary
This study identifies key proteins involved in Cordyceps polysaccharide biosynthesis, revealing that sugar metabolism, particularly galactose metabolism, is crucial during the logarithmic growth phase for producing these bioactive compounds.
Area of Science:
- Biochemistry
- Molecular Biology
- Mycology
Background:
- Cordyceps polysaccharides possess significant pharmacological properties, including immune modulation and anti-tumor effects.
- The biosynthetic pathways of these valuable compounds are not fully understood.
- Elucidating these mechanisms is crucial for metabolic regulation and potential applications.
Purpose of the Study:
- To identify differentially expressed proteins (DEPs) during Cordyceps polysaccharide biosynthesis.
- To understand the molecular mechanisms underlying polysaccharide production in Cordyceps.
- To provide a foundation for the metabolic engineering of Cordyceps polysaccharides.
Main Methods:
- Quantitative proteomics using TMT labeling to identify and quantify 1,242 DEPs.
- Comparative proteomic analysis across different growth phases.
- Bioinformatic annotation of DEPs and KEGG pathway analysis.
- Quantitative PCR (qPCR) to validate protein expression levels.
Main Results:
- Cordyceps polysaccharide biosynthesis is most active during the logarithmic growth phase, with significant involvement of sugar metabolism pathways like galactose metabolism.
- Key DEPs with glycosyltransferase activity were identified, playing a pivotal role in polysaccharide synthesis.
- Several proteins, including NOS, ID-RCD, Arp2, NAD-GDH, phaA, GGT, CYP53A1, LAD, lacZ, and AAO, were significantly upregulated.
- The lacZ gene, potentially via its glycosyltransferase activity, emerged as a critical factor in biosynthesis.
- Transcriptional data largely corroborated protein-level expression changes.
Conclusions:
- This research illuminates the protein landscape of Cordyceps polysaccharide biosynthesis.
- It highlights the importance of sugar metabolism and specific glycosyltransferases, such as those related to the lacZ gene, in the production process.
- The findings offer a scientific basis for optimizing Cordyceps polysaccharide production through metabolic regulation.
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