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Updated: Jan 13, 2026

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

Designing Functional Membranes with Tunable PDA/PEI Coatings for Enzyme Entrapment.

Sara Barricella¹, Magdalena Giergiel², Callum Gassner²

¹Bioresource Processing Research Institute of Australia (BioPRIA), Department of Chemical and Biological Engineering, Monash University, Clayton, Victoria 3800, Australia.

ACS Applied Materials & Interfaces

|January 8, 2026

Summary

Polydopamine (PDA) and polyethylenimine (PEI) co-adsorption creates enzyme-entrapped membranes. Optimizing deposition parameters tunes membrane performance for biocatalysis, achieving high enzyme retention without reducing permeance.

Keywords:

PEI Polydopamine enzyme membrane nanoscale analysis reverse filtration segregation

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Last Updated: Jan 13, 2026

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Area of Science:

Materials Science
Chemical Engineering
Biotechnology

Background:

Polydopamine (PDA) coatings enhance filtration membrane properties like hydrophilicity and biocompatibility.
Enzyme immobilization in membranes is crucial for biocatalysis but challenges exist with incompatible enzymes.

Purpose of the Study:

To fabricate enzyme-entrapped membranes by co-adsorbing PDA and polyethylenimine (PEI).
To investigate the effect of deposition parameters on membrane performance and enzyme retention.
To enable segregation of incompatible enzymes within a nanoporous membrane.

Main Methods:

Co-adsorption of PDA and PEI on nanoporous membranes under varying conditions (concentrations, volumes, pressures).
Evaluation of membrane permeance reduction and transaminase (TA) retention.
Application of Response Surface Methodology (RSM) for parameter optimization.
Characterization using Fourier-Transform Infrared Spectroscopy (FTIR) and Atomic Force Microscopy with Infrared Spectroscopy (AFM-IR).

Main Results:

PDA/PEI concentration significantly impacts permeance reduction and enzyme retention.
Nonlinear effects of solution volume and applied pressure, with significant interactions with concentration.
Increased polymer deposition and coating thickness observed with higher concentrations and longer residence times.
AFM-IR mapping confirmed correlations between deposition parameters, coating distribution, and permeance reduction.

Conclusions:

Membrane performance, including enzyme retention and permeance, can be precisely tuned by optimizing PDA/PEI co-adsorption parameters.
A robust framework combining RSM and nanoscale IR spectroscopy is established for designing and characterizing enzyme-entrapped membranes.
This approach offers a quantitative method for optimizing membrane-based biocatalysis applications.