Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

5.3K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.3K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

10.5K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Performance of Modified Cement-Based Slurry Incorporation with Multi-Walled Carbon Nanotubes (MWCNTs), Polycarboxylate Ether Superplasticizer (PCE) and Hydroxypropyl Methylcellulose (HPMC) Under High-Temperature.

Materials (Basel, Switzerland)·2026
Same author

ZnZrF<sub>6</sub>-Based Electrolytes are Used in High-Stability Zinc-Ion Capacitors.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Spatio-dipolar synergy modulated interfacial molecular bridge for calendar-aging-resistant aqueous zinc-ion batteries.

National science review·2026
Same author

Formulation-resolved VOC emission factors measured in a dynamic chamber and corresponding secondary formation potentials for selected architectural paints.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Coordinated sub-cycle modulation atomic layer deposition of atomically homogeneous GeTe<sub>9</sub> thin films for high-performance OTSs.

Materials horizons·2026
Same author

The impact of political climate on student health and wellbeing: a qualitative study.

BMC public health·2026

Related Experiment Video

Updated: Jan 13, 2026

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
09:52

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

Published on: January 6, 2023

2.7K

DAT-delivered astaxanthin reprograms adipogenesis through RhoGDI1 dephosphorylation at Ser174 and RhoA/FAK/ERK1/2

Yapeng Hou1, Yanhui Zhang1, Ziyu Ma1

  • 1Department of Tissue Engineering, School of Intelligent Medicine, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning, 110122, China.

Biomaterials
|January 8, 2026
PubMed
Summary

Astaxanthin (AST) enhances adipose-derived stem cell (ADSC) differentiation for tissue repair. This study reveals AST targets RhoGDI1, promoting adipogenesis via a novel pathway for adipose regeneration.

Keywords:
AdipogenesisAdipose-derived stem cellsAstaxanthinRhoA signaling pathwayRhoGDI1

More Related Videos

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.7K
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
11:11

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

2.7K

Related Experiment Videos

Last Updated: Jan 13, 2026

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
09:52

Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System

Published on: January 6, 2023

2.7K
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.7K
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
11:11

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

2.7K

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Cellular Biology

Background:

  • Adipose tissue is crucial for soft tissue defect repair, but its regenerative capacity is often limited.
  • Astaxanthin (AST) shows potential in ameliorating adipose dysfunction, yet its pro-adipogenic mechanisms are not fully understood.
  • Existing treatments face challenges due to inadequate adipogenic potential in clinical settings.

Purpose of the Study:

  • To develop an astaxanthin-loaded decellularized adipose tissue (DAT) hydrogel to enhance adipogenic potential.
  • To elucidate the underlying molecular mechanisms of AST's pro-adipogenic activity.
  • To investigate the therapeutic potential of DAT-AST hydrogels for adipose tissue regeneration.

Main Methods:

  • Development of an AST-loaded DAT hydrogel.
  • Subcutaneous implantation of DAT-AST hydrogel into rabbit inguinal fat pad defects.
  • In vitro studies using human adipose-derived stem cells (ADSCs) to assess adipogenic differentiation.
  • Investigation of AST's effect on RhoGDI1 phosphorylation and downstream signaling pathways (RhoA/FAK/ERK1/2).

Main Results:

  • DAT-AST hydrogel implantation significantly enhanced adipogenesis and tissue restoration in rabbit models.
  • AST significantly promoted adipogenic differentiation in ADSCs by suppressing RhoGDI1 phosphorylation at Ser174.
  • RhoGDI1 knockdown abolished AST-induced lipid accumulation and disrupted the RhoA/FAK/ERK1/2 signaling axis.

Conclusions:

  • RhoGDI1 dephosphorylation is a critical mechanism for AST's pro-adipogenic action.
  • This study identifies a targetable pathway for improving adipose regeneration.
  • DAT hydrogels represent a promising delivery platform for AST in therapeutic applications for adipose tissue repair.