Exosome-transmitted long noncoding RNA SNHG1 promotes prostate cancer bone metastasis via YBX1/MMP16 axis

Area of Science:

• Oncology
• Molecular Biology
• Biochemistry

Background:

• Prostate cancer (PCa) with bone metastasis has a poor prognosis.
• Exosomes mediate intercellular communication in the tumor microenvironment.
• The role of long noncoding RNA SNHG1 in PCa bone metastasis is unclear.

Purpose of the Study:

• Investigate the mechanisms of exosome-encapsulated SNHG1 in PCa bone metastasis.
• Determine SNHG1's role in osteogenic differentiation and PCa progression.

Main Methods:

• Analysis of SNHG1 expression in PCa tissues and exosomes.
• Functional experiments (gain- and loss-of-function) in vitro and in vivo.
• Mechanistic studies involving exosome transfer, protein binding, and gene transcription.

Main Results:

• SNHG1 is overexpressed in PCa, enriched in exosomes, and correlates with bone metastasis.
• Exosomal SNHG1 promotes osteogenic differentiation and extracellular matrix mineralization.
• Knockdown of exosomal SNHG1 suppresses PCa bone metastasis in vivo.
• Exosomal SNHG1 binds YBX1, promoting MMP16 transcription and translation.

Conclusions:

• PCa-derived exosomes carrying SNHG1 mediate osteogenesis via the SNHG1/YBX1/MMP16 axis.
• SNHG1 is a potential diagnostic marker and therapeutic target for PCa bone metastasis.