[A new jatrophane diterpenoid with anti-inflammatory activity from Euphorbia thymifolia]

The 95% ethanol extract of the dried aerial parts of Euphorbia thymifolia was separated and purified by various chromatographic techniques, including MCI column chromatography, ODS column chromatography, and high-performance liquid chromatography(HPLC). The structures of the isolated monomeric compounds were determined by spectroscopic methods such as UV, IR, 1D NMR, 2D NMR, and MS. Ten compounds were isolated, namely euphthymifolone A(1), euphoscopin F(2), helioscopid D(3), euphoscopin L(4), euphoscopin E(5), helioscopinolide B(6), 2α-hydroxy helioscopinolide B(7), helioscopinolide A(8), anabellamide(9), and aurantiamide(10). Among them, compounds 1-5 are jatrophane-type diterpenoids, compounds 6-8 are abietane-type diterpenoids, and compounds 9 and 10 are alkaloids. Compound 1 is a new compound, while compounds 9 and 10 were isolated from this genus for the first time. Compound 1 showed certain nitric oxide(NO) inhibitory activity in lipopolysaccharide(LPS)-induced BV-2 cells[IC_(50)=(65.4±1.36)μmol·L~(-1)].